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FKBP10 Rabbit mAb (A24072)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - FKBP10 Rabbit mAb (A24072)

Western blot analysis of various lysates using FKBP10 Rabbit mAb (A24072) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): A-431
Exposure time: 15s.

ABclonal:Immunofluorescence - FKBP10 Rabbit mAb (A24072)

Confocal imaging of HeLa cells using FKBP10 Rabbit mAb (A24072, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameFKBP10 Rabbit mAb
Catalog No.A24072
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC64550
The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported, but their biological validity has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 303-582 of human FKBP10 (NP_068758.3).
SequenceFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL
Gene ID
Swiss Prot
SynonymsFKBP10; BRKS1; FKBP65; OI11; OI6; PPIASE; hFKBP65; FK506 binding protein 10
Calculated MW39kDa/64kDa
Observed MW70kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, U-87 MG
Cellular locationEndoplasmic reticulum lumen

Documents

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    ABclonal:Western blot - FKBP10 Rabbit mAb (A24072)}

    Western blot - FKBP10 Rabbit mAb (A24072)

    Western blot analysis of various lysates using FKBP10 Rabbit mAb (A24072) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Negative control (NC): A-431
    Exposure time: 15s.
    ABclonal:Immunofluorescence - FKBP10 Rabbit mAb (A24072)}

    Immunofluorescence - FKBP10 Rabbit mAb (A24072)

    Confocal imaging of HeLa cells using FKBP10 Rabbit mAb (A24072, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)