제품 > 항체 > 다중클론항체(pAb)

FMR1NB Rabbit pAb (A5183)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FMR1NB Rabbit pAb (A5183)

Western blot analysis of extracts of various cell lines, using FMR1NB Antibody (A5183) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameFMR1NB Rabbit pAb
Catalog No.A5183
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be integral component of membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 90-183 of human FMR1NB (NP_689791.1).
SequenceLCSGSSYFVLANGHILPNSENAHGQSLEEDSALEALLNFFFPTTCNLRENQVAKPCNELQDLSESECLRHKCCFSSSGTTSFKCFAPFRDVPKQ
Gene ID
Swiss Prot
SynonymsCT37; NYSAR35; NY-SAR-35
Calculated MW29kDa
Observed MW29kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesNCI-H460, K-562, Mouse brain, Mouse liver, Rat liver, Rat brain
Cellular locationMembrane, Multi-pass membrane protein
ABclonal:Western blot - FMR1NB Rabbit pAb (A5183)}

Western blot - FMR1NB Rabbit pAb (A5183)

Western blot analysis of extracts of various cell lines, using FMR1NB Antibody (A5183) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)