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FOSL2 Rabbit pAb (A2729)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - FOSL2 Rabbit pAb (A2729)

Western blot analysis of various lysates using FOSL2 Rabbit pAb (A2729) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - FOSL2 Rabbit pAb (A2729)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using FOSL2 Rabbit pAb (A2729) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - FOSL2 Rabbit pAb (A2729)

Immunofluorescence analysis of U-2 OS cells using FOSL2 Rabbit pAb (A2729) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFOSL2 Rabbit pAb
Catalog No.A2729
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 187-326 of human FOSL2 (NP_005244.1).
SequenceHGPVCKISPEERRSPPAPGLQPMRSGGGSVGAVVVKQEPLEEDSPSSSSAGLDKAQRSVIKPISIAGGFYGEEPLHTPIVVTSTPAVTPGTSNLVFTYPSVLEQESPASPSESCSKAHRRSSSSGDQSSDSLNSPTLLAL
Gene ID
Swiss Prot
SynonymsFRA2; FOSL2
Calculated MW35kDa
Observed MW35kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBT-474, A-549
Cellular locationNucleus.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - FOSL2 Rabbit pAb (A2729)}

Western blot - FOSL2 Rabbit pAb (A2729)

Western blot analysis of various lysates using FOSL2 Rabbit pAb (A2729) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - FOSL2 Rabbit pAb (A2729)}

Immunohistochemistry - FOSL2 Rabbit pAb (A2729)

Immunohistochemistry analysis of paraffin-embedded Human esophagus using FOSL2 Rabbit pAb (A2729) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - FOSL2 Rabbit pAb (A2729)}

Immunofluorescence - FOSL2 Rabbit pAb (A2729)

Immunofluorescence analysis of U-2 OS cells using FOSL2 Rabbit pAb (A2729) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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