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FRMD6 Rabbit pAb (A9995)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FRMD6 Rabbit pAb (A9995)

Western blot analysis of various lysates using FRMD6 Rabbit pAb (A9995) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - FRMD6 Rabbit pAb (A9995)

Western blot analysis of various lysates using FRMD6 Rabbit pAb (A9995) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - FRMD6 Rabbit pAb (A9995)

Immunohistochemistry analysis of paraffin-embedded Rat heart using FRMD6 Rabbit pAb (A9995) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - FRMD6 Rabbit pAb (A9995)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using FRMD6 Rabbit pAb (A9995) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - FRMD6 Rabbit pAb (A9995)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using FRMD6 Rabbit pAb (A9995) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - FRMD6 Rabbit pAb (A9995)

Immunofluorescence analysis of C6 cells using FRMD6 Rabbit pAb (A9995) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FRMD6 Rabbit pAb (A9995)

Immunofluorescence analysis of U2OS cells using FRMD6 Rabbit pAb (A9995) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameFRMD6 Rabbit pAb
Catalog No.A9995
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in actomyosin structure organization. Predicted to act upstream of or within apical constriction; cellular protein localization; and regulation of actin filament-based process. Predicted to be located in apical junction complex. Predicted to be active in cytoskeleton.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 82-183 of human FRMD6 (NP_001253975.1).
SequenceKEWKKEASKVRQYEVTWGIDQFGPPMIIHFRVQYYVENGRLISDRAARYYYYWHLRKQVLHSQCVLREEAYFLLAAFALQADLGNFKRNKHYGKYFEPEAYF
Gene ID
Swiss Prot
SynonymsEX1; Willin; C14orf31; c14_5320; FRMD6
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesSH-SY5Y, HeLa, HepG2, MCF7, Mouse kidney, OVCAR8, Rat lung, Rat spleen
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein.

Documents

Certificate of Compliance

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ABclonal:Western blot - FRMD6 Rabbit pAb (A9995)}

Western blot - FRMD6 Rabbit pAb (A9995)

Western blot analysis of various lysates using FRMD6 Rabbit pAb (A9995) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - FRMD6 Rabbit pAb (A9995)}

Western blot - FRMD6 Rabbit pAb (A9995)

Western blot analysis of various lysates using FRMD6 Rabbit pAb (A9995) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - FRMD6 Rabbit pAb (A9995)}

Immunohistochemistry - FRMD6 Rabbit pAb (A9995)

Immunohistochemistry analysis of paraffin-embedded Rat heart using FRMD6 Rabbit pAb (A9995) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - FRMD6 Rabbit pAb (A9995)}

Immunohistochemistry - FRMD6 Rabbit pAb (A9995)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using FRMD6 Rabbit pAb (A9995) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - FRMD6 Rabbit pAb (A9995)}

Immunohistochemistry - FRMD6 Rabbit pAb (A9995)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using FRMD6 Rabbit pAb (A9995) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - FRMD6 Rabbit pAb (A9995)}

Immunofluorescence - FRMD6 Rabbit pAb (A9995)

Immunofluorescence analysis of C6 cells using FRMD6 Rabbit pAb (A9995) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FRMD6 Rabbit pAb (A9995)}

Immunofluorescence - FRMD6 Rabbit pAb (A9995)

Immunofluorescence analysis of U2OS cells using FRMD6 Rabbit pAb (A9995) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)