제품 > 항체 > 단일클론항체(mAb)

FRS2 Rabbit mAb (A22711)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Mouse kidney, using FRS2 Rabbit mAb (A22711) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - FRS2 Rabbit mAb (A22711)

Confocal imaging of THP-1 cells using FRS2 Rabbit mAb (A22711, dilution 1:900)(Red).DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product name	FRS2 Rabbit mAb
Catalog No.	A22711
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57141

Background

Enables fibroblast growth factor receptor binding activity and neurotrophin TRKA receptor binding activity. Involved in negative regulation of cardiac muscle cell differentiation. Acts upstream of or within fibroblast growth factor receptor signaling pathway. Located in adherens junction. Biomarker of renal cell carcinoma.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 110-120 of human FRS2. (NP_006645.3).
Sequence	INVVEEPVVER
Gene ID
Swiss Prot
Synonyms	SNT; SNT1; FRS1A; FRS2A; SNT-1; FRS2alpha; FRS2
Calculated MW	57kDa
Observed MW	80kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse kidney
Cellular location	Endomembrane system