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FTCD Rabbit mAb (A22654)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FTCD Rabbit mAb (A22654)

Western blot analysis of various lysates, using FTCD Rabbit mAb (A22654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - FTCD Rabbit mAb (A22654)

Western blot analysis of lysates from Mouse liver, using FTCD Rabbit mAb (A22654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - FTCD Rabbit mAb (A22654)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using FTCD Rabbit mAb (A22654) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FTCD Rabbit mAb (A22654)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using FTCD Rabbit mAb (A22654) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FTCD Rabbit mAb (A22654)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using FTCD Rabbit mAb (A22654) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameFTCD Rabbit mAb
Catalog No.A22654
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58229
The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 320-541 of human FTCD. (NP_006648.1).
SequenceRIIEYLVPERGPERGLGSKSLRAFVGEVGARSAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYLEAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE
Gene ID
Swiss Prot
SynonymsLCHC1; FTCD
Calculated MW59kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Mouse liver
Cellular locationCytoplasm, Golgi apparatus, centriole, centrosome, cytoskeleton, microtubule organizing center

Documents

Certificate of Compliance

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ABclonal:Western blot - FTCD Rabbit mAb (A22654)}

Western blot - FTCD Rabbit mAb (A22654)

Western blot analysis of various lysates, using FTCD Rabbit mAb (A22654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - FTCD Rabbit mAb (A22654)}

Western blot - FTCD Rabbit mAb (A22654)

Western blot analysis of lysates from Mouse liver, using FTCD Rabbit mAb (A22654) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - FTCD Rabbit mAb (A22654)}

Immunohistochemistry - FTCD Rabbit mAb (A22654)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using FTCD Rabbit mAb (A22654) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FTCD Rabbit mAb (A22654)}

Immunohistochemistry - FTCD Rabbit mAb (A22654)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using FTCD Rabbit mAb (A22654) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FTCD Rabbit mAb (A22654)}

Immunohistochemistry - FTCD Rabbit mAb (A22654)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using FTCD Rabbit mAb (A22654) at a dilution of 1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (3)

Secondary Antibodies (26)