제품 > 항체 > 다중클론항체(pAb)

FT Rabbit pAb (A21280)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Arabidopsis thaliana

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using FT Rabbit pAb (A21280) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product name	FT Rabbit pAb
Catalog No.	A21280
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

FT, together with LFY, promotes flowering and is antagonistic with its homologous gene, TERMINAL FLOWER1 (TFL1). FT is expressed in leaves and is induced by long day treatment. Either the FT mRNA or protein is translocated to the shoot apex where it induces its own expression. Recent data suggests that FT protein acts as a long-range signal. FT is a target of CO and acts upstream of SOC1.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 70-150 of arabidopsis thaliana FT (NP_176726.1).
Sequence	VDPDVPSPSNPHLREYLHWLVTDIPATTGTTFGNEIVCYENPSPTAGIHRVVFILFRQLGRQTVYAPGWRQNFNTREFAEI
Gene ID
Swiss Prot
Synonyms	F5I14.3; F5I14_3; FLOWERING LOCUS T; REDUCED STEM BRANCHING 8; RSB8; FT
Calculated MW	20kDa
Observed MW	20kDa

Applications

Reactivity	Arabidopsis thaliana
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Seedling, Before inflorescence
Cellular location	cytoplasm, endoplasmic reticulum, nucleus