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FUBP1 Rabbit mAb (A9077)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FUBP1 Rabbit mAb (A9077)

Western blot analysis of various lysates using FUBP1 Rabbit mAb (A9077) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded human thyroid tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded human breast cancer tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat liver tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse lung tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded human esophagus tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat intestine tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat lung tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse spleen tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse intestin tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - FUBP1 Rabbit mAb (A9077)

Confocal imaging of U-2 OS cells using FUBP1 Rabbit mAb (A9077, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - FUBP1 Rabbit mAb (A9077)

Confocal imaging of paraffin-embedded Human breast cancer tissue using FUBP1 Rabbit mAb (A9077, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - FUBP1 Rabbit mAb (A9077)

Confocal imaging of HeLa cells using FUBP1 Rabbit mAb (A9077, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Overview

Product nameFUBP1 Rabbit mAb
Catalog No.A9077
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1403
The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 545-644 of human FUBP1 (Q96AE4).
SequenceYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQGQ
Gene ID
Swiss Prot
SynonymsFBP; FUBP; hDH V; FUBP1
Calculated MW68kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCytoplasm, Nucleoplasm, Nnucleus

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ABclonal:Western blot - FUBP1 Rabbit mAb (A9077)}

Western blot - FUBP1 Rabbit mAb (A9077)

Western blot analysis of various lysates using FUBP1 Rabbit mAb (A9077) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded human thyroid tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded human breast cancer tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat liver tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse lung tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded human esophagus tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat intestine tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded rat lung tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse spleen tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - FUBP1 Rabbit mAb (A9077)}

Immunohistochemistry - FUBP1 Rabbit mAb (A9077)

Immunohistochemistry analysis of FUBP1 in paraffin-embedded mouse intestin tissue using FUBP1 Rabbit mAb (A9077) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - FUBP1 Rabbit mAb (A9077)}

Immunofluorescence - FUBP1 Rabbit mAb (A9077)

Confocal imaging of U-2 OS cells using FUBP1 Rabbit mAb (A9077, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Immunofluorescence - FUBP1 Rabbit mAb (A9077)}

Immunofluorescence - FUBP1 Rabbit mAb (A9077)

Confocal imaging of paraffin-embedded Human breast cancer tissue using FUBP1 Rabbit mAb (A9077, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - FUBP1 Rabbit mAb (A9077)}

Immunofluorescence - FUBP1 Rabbit mAb (A9077)

Confocal imaging of HeLa cells using FUBP1 Rabbit mAb (A9077, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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