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FUBP1 Rabbit pAb (A5587)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - FUBP1 Rabbit pAb (A5587)

Western blot analysis of various lysates using FUBP1 Rabbit pAb (A5587) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - FUBP1 Rabbit pAb (A5587)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using FUBP1 Rabbit pAb (A5587) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FUBP1 Rabbit pAb (A5587)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using FUBP1 Rabbit pAb (A5587) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FUBP1 Rabbit pAb (A5587)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FUBP1 Rabbit pAb (A5587) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - FUBP1 Rabbit pAb (A5587)

Immunofluorescence analysis of C6 cells using FUBP1 Rabbit pAb (A5587) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - FUBP1 Rabbit pAb (A5587)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg FUBP1 antibody (A5587). Western blot was performed from the immunoprecipitate using FUBP1 antibody (A5587) at a dilution of 1:1000.

Overview

Product nameFUBP1 Rabbit pAb
Catalog No.A5587
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a single stranded DNA-binding protein that binds to multiple DNA elements, including the far upstream element (FUSE) located upstream of c-myc. Binding to FUSE occurs on the non-coding strand, and is important to the regulation of c-myc in undifferentiated cells. This protein contains three domains, an amphipathic helix N-terminal domain, a DNA-binding central domain, and a C-terminal transactivation domain that contains three tyrosine-rich motifs. The N-terminal domain is thought to repress the activity of the C-terminal domain. This protein is also thought to bind RNA, and contains 3'-5' helicase activity with in vitro activity on both DNA-DNA and RNA-RNA duplexes. Aberrant expression of this gene has been found in malignant tissues, and this gene is important to neural system and lung development. Binding of this protein to viral RNA is thought to play a role in several viral diseases, including hepatitis C and hand, foot and mouth disease. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 302-644 of human FUBP1 (NP_003893.2).
SequenceQNDAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQGQ
Gene ID
Swiss Prot
SynonymsFBP; FUBP; hDH V; FUBP1
Calculated MW68kDa
Observed MW79kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:100
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesU-87MG, HeLa, HepG2, THP-1, Mouse brain
Cellular locationNucleus
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - FUBP1 Rabbit pAb (A5587)}

Western blot - FUBP1 Rabbit pAb (A5587)

Western blot analysis of various lysates using FUBP1 Rabbit pAb (A5587) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - FUBP1 Rabbit pAb (A5587)}

Immunohistochemistry - FUBP1 Rabbit pAb (A5587)

Immunohistochemistry analysis of paraffin-embedded Human liver damage using FUBP1 Rabbit pAb (A5587) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FUBP1 Rabbit pAb (A5587)}

Immunohistochemistry - FUBP1 Rabbit pAb (A5587)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using FUBP1 Rabbit pAb (A5587) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FUBP1 Rabbit pAb (A5587)}

Immunohistochemistry - FUBP1 Rabbit pAb (A5587)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FUBP1 Rabbit pAb (A5587) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - FUBP1 Rabbit pAb (A5587)}

Immunofluorescence - FUBP1 Rabbit pAb (A5587)

Immunofluorescence analysis of C6 cells using FUBP1 Rabbit pAb (A5587) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - FUBP1 Rabbit pAb (A5587)}

Immunoprecipitation - FUBP1 Rabbit pAb (A5587)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg FUBP1 antibody (A5587). Western blot was performed from the immunoprecipitate using FUBP1 antibody (A5587) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)