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FUT4 Rabbit pAb (A16320)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - FUT4 Rabbit pAb (A16320)

Immunohistochemistry analysis of paraffin-embedded Human colon using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - FUT4 Rabbit pAb (A16320)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - FUT4 Rabbit pAb (A16320)

Immunofluorescence analysis of paraffin-embedded human colon carcinoma using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - FUT4 Rabbit pAb (A16320)

Immunofluorescence analysis of paraffin-embedded human colon carcinoma using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFUT4 Rabbit pAb
Catalog No.A16320
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 186-331 of human FUT4 (NP_002024.1).
SequenceSRPVGVLLWWEPFGGRDSAPRPPPDCRLRFNISGCRLLTDRASYGEAQAVLFHHRDLVKGPPDWPPPWGIQAHTAEEVDLRVLDYEEAAAAAEALATSSPRPPGQRWVWMNFESPSHSPGLRSLASNLFNWTLSYRADSDVFVPYG
Gene ID
Swiss Prot
SynonymsLeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; FUT4
Calculated MW59kDa
Observed MW59kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHL-60
Cellular locationGolgi apparatus, Golgi stack membrane, Single-pass type II membrane protein.
Customer validation

IHC(Homo sapiens)

IF(Gallus gallus f. domestica)

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Immunohistochemistry - FUT4 Rabbit pAb (A16320)}

Immunohistochemistry - FUT4 Rabbit pAb (A16320)

Immunohistochemistry analysis of paraffin-embedded Human colon using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - FUT4 Rabbit pAb (A16320)}

Immunohistochemistry - FUT4 Rabbit pAb (A16320)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - FUT4 Rabbit pAb (A16320)}

Immunofluorescence - FUT4 Rabbit pAb (A16320)

Immunofluorescence analysis of paraffin-embedded human colon carcinoma using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - FUT4 Rabbit pAb (A16320)}

Immunofluorescence - FUT4 Rabbit pAb (A16320)

Immunofluorescence analysis of paraffin-embedded human colon carcinoma using FUT4 Rabbit pAb (A16320) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A16320? Please let us know so that we can cite the reference in this datasheet.

항체 (3)

Secondary Antibodies (26)