제품 > 항체 > 다중클론항체(pAb)

Fam221a Rabbit pAb (A20273)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Fam221a Rabbit pAb (A20273)

Western blot analysis of various lysates using Fam221a Rabbit pAb (A20273) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Fam221a Rabbit pAb (A20273)

Immunofluorescence analysis of A-549 cells using Fam221a Rabbit pAb (A20273) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Fam221a Rabbit pAb (A20273)

Immunofluorescence analysis of C6 cells using Fam221a Rabbit pAb (A20273) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameFam221a Rabbit pAb
Catalog No.A20273
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Orthologous to human FAM221A (family with sequence similarity 221 member A).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-292 of mouse Fam221a (NP_766315.1).
SequenceMERLTLPPGGAEAVDEYLEYRRIVGEDDGGKLFTPEEYEEYKKKVLPMRLQNRLFVSWRSPTGMDCKLVGPETLCFCTHRYKQHKTDFETIPQQRPIALPCRVSGCRCKAYHYVPLNGTQPIRCRCKHFADQHSAALGFTCNACSKCSGFHSCYTCACGQPAYAHDTVVETRQERLAQGKPVGRDVPYAAMGGLTGFSSLAEGYMRLDDSGIGAPSVEVLDSAVSAMDHPFLRAMHAPSTSSPQPLAGGNEVGPSTQLSSLRKPEEDDMAYFERQYQERIKLEKAAKQKGKV
Gene ID
Swiss Prot
SynonymsD330028D13Rik; Fam221a
Calculated MW33kDa
Observed MW33kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis, Rat testis
Cellular locationCytoplasm

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Fam221a Rabbit pAb (A20273)}

    Western blot - Fam221a Rabbit pAb (A20273)

    Western blot analysis of various lysates using Fam221a Rabbit pAb (A20273) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - Fam221a Rabbit pAb (A20273)}

    Immunofluorescence - Fam221a Rabbit pAb (A20273)

    Immunofluorescence analysis of A-549 cells using Fam221a Rabbit pAb (A20273) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - Fam221a Rabbit pAb (A20273)}

    Immunofluorescence - Fam221a Rabbit pAb (A20273)

    Immunofluorescence analysis of C6 cells using Fam221a Rabbit pAb (A20273) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)