제품 > 항체 > 다중클론항체(pAb)

Fam50b Rabbit pAb (A20245)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Fam50b Rabbit pAb (A20245)

Western blot analysis of lysates from Mouse testis, using Fam50b Rabbit pAb (A20245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - Fam50b Rabbit pAb (A20245)

Western blot analysis of lysates from Rat testis, using Fam50b Rabbit pAb (A20245) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Fam50b Rabbit pAb (A20245)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Fam50b Rabbit pAb (A20245) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Fam50b Rabbit pAb (A20245)

Immunohistochemistry analysis of paraffin-embedded mouse lung using Fam50b Rabbit pAb (A20245) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Fam50b Rabbit pAb (A20245)

Immunohistochemistry analysis of paraffin-embedded rat lung using Fam50b Rabbit pAb (A20245) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameFam50b Rabbit pAb
Catalog No.A20245
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in chromatin organization. Predicted to be located in intercellular bridge; midbody; and nucleoplasm. Predicted to be active in nucleus. Is expressed in primary spermatocyte; seminiferous tubule; and spermatid. Orthologous to human FAM50B (family with sequence similarity 50 member B).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 70-150 of mouse Fam50b (NP_620085.2).
SequenceLNDMKAKQEALLREREMQLAKREQLEQRRIQLEMLREKERRRERKRKISNLSFTLDEEEGDQEDSRQAESAEAHSAGAKKN
Gene ID
Swiss Prot
SynonymsX5L; D0H6S2654E; XAP-5-like; Fam50b
Calculated MW40kDa
Observed MW39kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse testis, Rat testis
Cellular locationintercellular bridge, nucleoplasm, nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - Fam50b Rabbit pAb (A20245)}

    Western blot - Fam50b Rabbit pAb (A20245)

    Western blot analysis of lysates from Mouse testis, using Fam50b Rabbit pAb (A20245) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Western blot - Fam50b Rabbit pAb (A20245)}

    Western blot - Fam50b Rabbit pAb (A20245)

    Western blot analysis of lysates from Rat testis, using Fam50b Rabbit pAb (A20245) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Immunohistochemistry - Fam50b Rabbit pAb (A20245)}

    Immunohistochemistry - Fam50b Rabbit pAb (A20245)

    Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Fam50b Rabbit pAb (A20245) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - Fam50b Rabbit pAb (A20245)}

    Immunohistochemistry - Fam50b Rabbit pAb (A20245)

    Immunohistochemistry analysis of paraffin-embedded mouse lung using Fam50b Rabbit pAb (A20245) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - Fam50b Rabbit pAb (A20245)}

    Immunohistochemistry - Fam50b Rabbit pAb (A20245)

    Immunohistochemistry analysis of paraffin-embedded rat lung using Fam50b Rabbit pAb (A20245) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)