제품 > 항체 > 단일클론항체(mAb)

Ferritin Light Chain Rabbit mAb (A11241)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from wild type (WT) and Ferritin Light Chain knockdown (KD) 293T cells using Ferritin Light Chain Rabbit mAb (A11241) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of various lysates using Ferritin Light Chain Rabbit mAb (A11241) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from Rat liver using Ferritin Light Chain Rabbit mAb (A11241) at 1:2000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Review

Overview

Product name	Ferritin Light Chain Rabbit mAb
Catalog No.	A11241
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC57605

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human Ferritin Light Chain (NP_000137.2).
Sequence	MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD
Gene ID
Swiss Prot
Synonyms	LFTD; NBIA3; Ferritin Light Chain
Calculated MW	20kDa
Observed MW	20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:9000 IHC-P 1:400 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	Rat liver, 293T, Mouse liver, Mouse ovary, Hep G2, SH-SY5Y
Cellular location	cytoplasm, cytosol, extracellular exosome, extracellular region.
Customer validation	(293T、HeLa) WB(Rattus norvegicus, Gallus gallus, Mus musculus, Homo sapiens, Mus musculus) IHC(Homo sapiens, Homo sapiens Rattus norvegicus) IHC(Homo sapiens, Mus musculus, Rattus norvegicus) ELISA(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

Ferritin Light Chain Rabbit mAb images

Western blot - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

J Inflamm Res

Application:

IF:

6.922

Species:

Rattus norvegicus

PMID:

34703276

Title:

Ferroptosis is Involved in Hyperoxic Lung Injury in Neonatal Rats

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Free radical biology & medicine

Application:

IF:

8.1

Species:

Gallus gallus

PMID:

35675856

Title:

Mercuric chloride induces sequential activation of ferroptosis and necroptosis in chicken embryo kidney cells by triggering ferritinophagy

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Chemico-biological interactions

Application:

IF:

5.16

Species:

Mus musculus

PMID:

35661779

Title:

Ferroptosis is involved in the benzene-induced hematotoxicity in mice via iron metabolism, oxidative stress and NRF2 signaling pathway

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Mus musculus

PMID:

36341347

Title:

MiR-93-5p promotes granulosa cell apoptosis and ferroptosis by the NF-kB signaling pathway in polycystic ovary syndrome - PMC

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Aging

Application:

IHC

IF:

5.95

Species:

Homo sapiens

PMID:

37354485

Title:

Construction of a T-cell exhaustion-related gene signature for predicting prognosis and immune response in hepatocellular carcinoma - PMC

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

5.57

Species:

Mus musculus

PMID:

36925038

Title:

Deoxynivalenol induces testicular ferroptosis by regulating the Nrf2/System Xc−/GPX4 axis

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Neural regeneration research

Application:

IF:

6.05

Species:

Mus musculus

PMID:

36018179

Title:

Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Nature communications

Application:

WB, IHC

IF:

17.69

Species:

Homo sapiens, Mus musculus

PMID:

36463308

Title:

Non-classical ferroptosis inhibition by a small molecule targeting PHB2 - PMC

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Human reproduction (Oxford, England)

Application:

IHC

IF:

6.35

Species:

Homo sapiens Rattus norvegicus

PMID:

37814907

Title:

The humanin analogue (HNG) alleviates intrauterine adhesions by inhibiting endometrial epithelial cells ferroptosis: a rat model-based study

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Frontiers in molecular biosciences

Application:

ELISA

IF:

Species:

Homo sapiens

PMID:

38449697

Title:

Iron-induced kidney cell damage: insights into molecular mechanisms and potential diagnostic significance of urinary FTL - PMC

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Food Funct

Application:

WB, IHC

IF:

6.317

Species:

Rattus norvegicus

PMID:

34825691

Title:

Sesamin attenuates PM2. 5-induced cardiovascular injury by inhibiting ferroptosis in rats

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Apoptosis : an international journal on programmed cell death

Application:

IF:

7.2

Species:

Homo sapiens

PMID:

38553613

Title:

Deubiquitinating enzyme OTUD4 stabilizes RBM47 to induce ATF3 transcription: a novel mechanism underlying the restrained malignant properties of ccRCC cells

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

BMC complementary medicine and therapies

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

38632548

Title:

Study on the differential hepatotoxicity of raw polygonum multiflorum and polygonum multiflorum praeparata and its mechanism - PMC

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Environmental pollution (Barking, Essex : 1987)

Application:

IF:

8.9

Species:

Mus musculus

PMID:

38423272

Title:

Inhalation exposure to polystyrene nanoplastics induces chronic obstructive pulmonary disease-like lung injury in mice through multi-dimensional assessment

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Biological trace element research

Application:

IF:

3.9

Species:

Gallus gallus

PMID:

38502261

Title:

Dietary Iron Overload Triggers Hepatic Metabolic Disorders and Inflammation in Laying Hen

References for Ferritin Light Chain Rabbit mAb (A11241)

Product:

Ferritin Light Chain Rabbit mAb

Journal:

Frontiers in neuroscience

Application:

IF:

4.3

Species:

Mus musculus

PMID:

39315073

Title:

8-weeks aerobic exercise ameliorates cognitive deficit and mitigates ferroptosis triggered by iron overload in the prefrontal cortex of APPSwe/PSEN1dE9 mice through Xc-/GPx4 pathway - PMC