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Fetuin A/AHSG Rabbit mAb (A19264)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Fetuin A/AHSG Rabbit mAb (A19264)

Western blot analysis of extracts of Human plasma, using Fetuin A/AHSG antibody (A19264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Overview

Product nameFetuin A/AHSG Rabbit mAb
Catalog No.A19264
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2433
The protein encoded by this gene is a negatively-charged serum glycoprotein that is synthesized by hepatocytes. The encoded protein consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several processes, including endocytosis, brain development, and the formation of bone tissue. Defects in this gene are a cause of susceptibility to leanness.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human Fetuin A/AHSG (P02765).
SequenceMKSLVLLLCLAQLWGCHSAPHGPGLIYRQPNCDDPETEEAALVAIDYINQNLPWGYKHTLNQIDEVKVWPQQPSGELFEIEIDTLETTCHVLDPTPVARC
Gene ID
Swiss Prot
SynonymsAHS; A2HS; HSGA; APMR1; FETUA
Calculated MW39kDa
Observed MW50kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHuman plasma
Cellular locationSecreted

Documents

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ABclonal:Western blot - Fetuin A/AHSG Rabbit mAb (A19264)}

Western blot - Fetuin A/AHSG Rabbit mAb (A19264)

Western blot analysis of extracts of Human plasma, using Fetuin A/AHSG antibody (A19264) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

* For research use only. Not for therapeutic or diagnostic purposes.

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