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G0S2 Rabbit pAb (A9970)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - G0S2 Rabbit pAb (A9970)

Western blot analysis of extracts of various cell lines, using G0S2 Antibody (A9970) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameG0S2 Rabbit pAb
Catalog No.A9970
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Involved in extrinsic apoptotic signaling pathway and positive regulation of extrinsic apoptotic signaling pathway. Located in mitochondrion.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human G0S2 (NP_056529.1).
SequenceMETVQELIPLAKEMMAQKRKGKMVKLYVLGSVLALFGVVLGLMETVCSPFTAARRLRDQEAAVAELQAALERQALQKQALQEKGKQQDTVLGGRALSNRQ
Gene ID
Swiss Prot
SynonymsG0S2
Calculated MW11kDa
Observed MW11kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:100 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse lung, Mouse kidney
Cellular locationMitochondrion
Customer validation

WB(Homo sapiens, Mus musculus)

Documents

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    ABclonal:Western blot - G0S2 Rabbit pAb (A9970)}

    Western blot - G0S2 Rabbit pAb (A9970)

    Western blot analysis of extracts of various cell lines, using G0S2 Antibody (A9970) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A9970? Please let us know so that we can cite the reference in this datasheet.

    항체 (1)

    Secondary Antibodies (26)