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GALNT3 Rabbit pAb (A13985)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GALNT3 Rabbit pAb (A13985)

Western blot analysis of various lysates using GALNT3 Rabbit pAb (A13985) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GALNT3 Rabbit pAb (A13985)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GALNT3 Rabbit pAb (A13985) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GALNT3 Rabbit pAb (A13985)

Immunofluorescence analysis of U-2 OS cells using GALNT3 Rabbit pAb (A13985) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGALNT3 Rabbit pAb
Catalog No.A13985
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes UDP-GalNAc transferase 3, a member of the GalNAc-transferases family. This family transfers an N-acetyl galactosamine to the hydroxyl group of a serine or threonine residue in the first step of O-linked oligosaccharide biosynthesis. Individual GalNAc-transferases have distinct activities and initiation of O-glycosylation is regulated by a repertoire of GalNAc-transferases. The protein encoded by this gene is highly homologous to other family members, however the enzymes have different substrate specificities.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 43-160 of human GALNT3 (NP_004473.2).
SequenceVQYSKEESRMERNMKNKNKMLDLMLEAVNNIKDAMPKMQIGAPVRQNIDAGERPCLQGYYTAAELKPVLDRPPQDSNAPGASGKAFKTTNLSVEEQKEKERGEAKHCFNAFASDRISL
Gene ID
Swiss Prot
SynonymsHHS; HFTC; HFTC1; GalNAc-T3; GALNT3
Calculated MW73kDa
Observed MW73kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesBxPC-3, Rat testis
Cellular locationGolgi apparatus, Golgi stack membrane, Single-pass type II membrane protein

Documents

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    ABclonal:Western blot - GALNT3 Rabbit pAb (A13985)}

    Western blot - GALNT3 Rabbit pAb (A13985)

    Western blot analysis of various lysates using GALNT3 Rabbit pAb (A13985) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - GALNT3 Rabbit pAb (A13985)}

    Immunohistochemistry - GALNT3 Rabbit pAb (A13985)

    Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using GALNT3 Rabbit pAb (A13985) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
    ABclonal:Immunofluorescence - GALNT3 Rabbit pAb (A13985)}

    Immunofluorescence - GALNT3 Rabbit pAb (A13985)

    Immunofluorescence analysis of U-2 OS cells using GALNT3 Rabbit pAb (A13985) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)