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GATA2 Rabbit pAb (A0677)

Reviews (2)Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GATA2 Rabbit pAb (A0677)

Western blot analysis of lysates from HeLa cells, using GATA2 Rabbit pAb (A0677) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - GATA2 Rabbit pAb (A0677)

Immunohistochemistry analysis of paraffin-embedded mouse brain using GATA2 Rabbit pAb (A0677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GATA2 Rabbit pAb (A0677)

Immunohistochemistry analysis of paraffin-embedded rat brain using GATA2 Rabbit pAb (A0677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for GATA2 Rabbit pAb(A0677)
Review
ABclonal: review for GATA2 Rabbit pAb(A0677)

Overview

Product nameGATA2 Rabbit pAb
Catalog No.A0677
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GATA2 (NP_116027.2).
SequenceMEVAPEQPRWMAHPAVLNAQHPDSHHPGLAHNYMEPAQLLPPDEVDVFFNHLDSQGNPYYANPAHARARVSYSPAHARLTGGQMCRPHLLHSPGLPWLDGGKAALSAAAAHHHNPWTVSPFSKTPLHPSAAGGPGGPLSVYPGAGGGSGGGSGSSVASLTPTAAHSGSHLFGFPPTPPKEVSPDPSTTGAASPASSSAGGSAARGEDKDGVKYQVSLTESMKMESGSPLRPGLATMGTQPATHHPIPTYPSYVPAAAHDYSSGLFHPGGFLGGPASSFTPKQRSKARSCSEGRECVNCGA
Gene ID
Swiss Prot
SynonymsDCML; IMD21; NFE1B; MONOMAC; GATA2
Calculated MW51kDa
Observed MW51kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationNucleus
Customer validation

(SK-N-SH)

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - GATA2 Rabbit pAb (A0677)}

Western blot - GATA2 Rabbit pAb (A0677)

Western blot analysis of lysates from HeLa cells, using GATA2 Rabbit pAb (A0677) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - GATA2 Rabbit pAb (A0677)}

Immunohistochemistry - GATA2 Rabbit pAb (A0677)

Immunohistochemistry analysis of paraffin-embedded mouse brain using GATA2 Rabbit pAb (A0677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GATA2 Rabbit pAb (A0677)}

Immunohistochemistry - GATA2 Rabbit pAb (A0677)

Immunohistochemistry analysis of paraffin-embedded rat brain using GATA2 Rabbit pAb (A0677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)