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GATM Rabbit mAb (A24126)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GATM Rabbit mAb (A24126)

Western blot analysis of various lysates using GATM Rabbit mAb (A24126) at 1:5500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GATM Rabbit mAb (A24126)

Confocal imaging of paraffin-embedded Mouse kidney using GATM Rabbit mAb (A24126, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - GATM Rabbit mAb (A24126)

Confocal imaging of paraffin-embedded Rat kidney using GATM Rabbit mAb (A24126, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameGATM Rabbit mAb
Catalog No.A24126
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65368
This gene encodes a mitochondrial enzyme that belongs to the amidinotransferase family. This enzyme is involved in creatine biosynthesis, whereby it catalyzes the transfer of a guanido group from L-arginine to glycine, resulting in guanidinoacetic acid, the immediate precursor of creatine. Mutations in this gene cause arginine:glycine amidinotransferase deficiency, an inborn error of creatine synthesis characterized by cognitive disability, language impairment, and behavioral disorders.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 38-423 of human GATM (NP_001473.1).
Sequence STQAATASSRNSCAADDKATEPLPKDCPVSSYNEWDPLEEVIVGRAENACVPPFTIEVKANTYEKYWPFYQKQGGHYFPKDHLKKAVAEIEEMCNILKTEGVTVRRPDPIDWSLKYKTPDFESTGLYSAMPRDILIVVGNEIIEAPMAWRSRFFEYRAYRSIIKDYFHRGAKWTTAPKPTMADELYNQDYPIHSVEDRHKLAAQGKFVTTEFEPCFDAADFIRAGRDIFAQRSQVTNYLGIEWMRRHLAPDYRVHIISFKDPNPMHIDATFNIIGPGIVLSNPDRPCHQIDLFKKAGWTIITPPTPIIPDDHPLWMSSKWLSMNVLMLDEKRVMVDANEVPIQKMFEKLGITTIKVNIRNANSLGGGFHCWTCDVRRRGTLQSYLD
Gene ID
Swiss Prot
SynonymsAT; RFS; AGAT; FRTS; CCDS3; FRTS1; GATM
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:5000 - 1:30000
  • IHC-P 1:400 - 1:4000
  • IF/ICC 1:300 - 1:1200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse kidney, Rat kidney
Cellular locationCytoplasm, Intermembrane side, Mitochondrion inner membrane, Peripheral membrane protein.

Documents

Certificate of Compliance

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ABclonal:Western blot - GATM Rabbit mAb (A24126)}

Western blot - GATM Rabbit mAb (A24126)

Western blot analysis of various lysates using GATM Rabbit mAb (A24126) at 1:5500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)}

Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)}

Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)}

Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GATM Rabbit mAb (A24126)}

Immunohistochemistry - GATM Rabbit mAb (A24126)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using GATM Rabbit mAb (A24126) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - GATM Rabbit mAb (A24126)}

Immunofluorescence - GATM Rabbit mAb (A24126)

Confocal imaging of paraffin-embedded Mouse kidney using GATM Rabbit mAb (A24126, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - GATM Rabbit mAb (A24126)}

Immunofluorescence - GATM Rabbit mAb (A24126)

Confocal imaging of paraffin-embedded Rat kidney using GATM Rabbit mAb (A24126, dilution 1:300) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500)(Red).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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