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GCN2 Rabbit pAb (A12618)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GCN2 Rabbit pAb (A12618)

Western blot analysis of lysates from HepG2 cells, using GCN2 Rabbit pAb (A12618) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - GCN2 Rabbit pAb (A12618)

Immunohistochemistry analysis of paraffin-embedded rat kidney using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GCN2 Rabbit pAb (A12618)

Immunohistochemistry analysis of paraffin-embedded rat ovary using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GCN2 Rabbit pAb (A12618)

Immunofluorescence analysis of NIH/3T3 cells using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GCN2 Rabbit pAb (A12618)

Immunofluorescence analysis of PC-12 cells using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GCN2 Rabbit pAb (A12618)

Immunofluorescence analysis of U2OS cells using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGCN2 Rabbit pAb
Catalog No.A12618
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCN2 (NP_001013725.2).
SequenceMAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNAL
Gene ID
Swiss Prot
SynonymsGCN2; PVOD2
Calculated MW187kDa
Observed MW187kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2
Cellular locationcytosol, cytosolic ribosome
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - GCN2 Rabbit pAb (A12618)}

Western blot - GCN2 Rabbit pAb (A12618)

Western blot analysis of lysates from HepG2 cells, using GCN2 Rabbit pAb (A12618) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - GCN2 Rabbit pAb (A12618)}

Immunohistochemistry - GCN2 Rabbit pAb (A12618)

Immunohistochemistry analysis of paraffin-embedded rat kidney using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GCN2 Rabbit pAb (A12618)}

Immunohistochemistry - GCN2 Rabbit pAb (A12618)

Immunohistochemistry analysis of paraffin-embedded rat ovary using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GCN2 Rabbit pAb (A12618)}

Immunofluorescence - GCN2 Rabbit pAb (A12618)

Immunofluorescence analysis of NIH/3T3 cells using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GCN2 Rabbit pAb (A12618)}

Immunofluorescence - GCN2 Rabbit pAb (A12618)

Immunofluorescence analysis of PC-12 cells using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GCN2 Rabbit pAb (A12618)}

Immunofluorescence - GCN2 Rabbit pAb (A12618)

Immunofluorescence analysis of U2OS cells using GCN2 Rabbit pAb (A12618) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12618? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

Secondary Antibodies (26)