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Phospho-GCN2-T899 Rabbit mAb (AP1356)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - Phospho-GCN2-T899 Rabbit mAb (AP1356)

Western blot analysis of various lysates, using Phospho-GCN2-T899 Rabbit mAb (AP1356) at1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-GCN2-T899 Rabbit mAb
Catalog No.AP1356
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57177
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
ImmunogenA synthetic phosphorylated peptide around T899 of human Phospho-GCN2-T899 (NP_001013725.2).
SequenceHLTGM
Gene ID
Swiss Prot
SynonymsGCN2; PVOD2; Phospho-GCN2-T899
Calculated MW187kDa
Observed MW220kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa treated by Calyculin A
Cellular locationCytosol, Cytosolic ribosome.

Documents

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ABclonal:Western blot - Phospho-GCN2-T899 Rabbit mAb (AP1356)}

Western blot - Phospho-GCN2-T899 Rabbit mAb (AP1356)

Western blot analysis of various lysates, using Phospho-GCN2-T899 Rabbit mAb (AP1356) at1:2000 dilution. HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

* For research use only. Not for therapeutic or diagnostic purposes.

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