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GCSH Rabbit pAb (A13695)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GCSH Rabbit pAb (A13695)

Western blot analysis of various lysates, using GCSH Rabbit pAb (A13695) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)

Immunofluorescence analysis of C6 cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)

Immunofluorescence analysis of L929 cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)

Immunofluorescence analysis of U-2 OS cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)

Confocal immunofluorescence analysis of U2OS cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGCSH Rabbit pAb
Catalog No.A13695
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the H protein, which transfers the methylamine group of glycine from the P protein to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH). Two transcript variants, one protein-coding and the other probably not protein-coding, have been found for this gene. Also, several transcribed and non-transcribed pseudogenes of this gene exist throughout the genome.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human GCSH (NP_004474.2).
SequenceMALRVVRSVRALLCTLRAVPSPAAPCPPRPWQLGVGAVRTLRTGPALLSVRKFTEKHEWVTTENGIGTVGISNFAQEALGDVVYCSLPEVGTKLNKQDEFGALESVKAASELYSPLSGEVTEINEALAENPGLVNKSCYEDGWLIKMTLSNPSELDELMSEEAYEKYIKSIEE
Gene ID
Swiss Prot
SynonymsGCE; NKH; GCSH
Calculated MW19kDa
Observed MW15kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse kidney, Rat kidney
Cellular locationMitochondrion.
Customer validation

IHC(Homo sapiens)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - GCSH Rabbit pAb (A13695)}

Western blot - GCSH Rabbit pAb (A13695)

Western blot analysis of various lysates, using GCSH Rabbit pAb (A13695) at 1:700 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)}

Immunofluorescence - GCSH Rabbit pAb (A13695)

Immunofluorescence analysis of C6 cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)}

Immunofluorescence - GCSH Rabbit pAb (A13695)

Immunofluorescence analysis of L929 cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)}

Immunofluorescence - GCSH Rabbit pAb (A13695)

Immunofluorescence analysis of U-2 OS cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GCSH Rabbit pAb (A13695)}

Immunofluorescence - GCSH Rabbit pAb (A13695)

Confocal immunofluorescence analysis of U2OS cells using GCSH Rabbit pAb (A13695) at dilution of 1:100 (60x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13695? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

Secondary Antibodies (26)