제품 > 항체 > 다중클론항체(pAb)

GF14b Rabbit pAb (A20625)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Oryza sativa

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GF14b Rabbit pAb (A20625) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	GF14b Rabbit pAb
Catalog No.	A20625
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes.

Immunogen information

Immunogen	Recombinant protein of Oryza sativa GF14b.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	GF14B; B0812A04.1; GF14b
Calculated MW	30kDa
Observed MW	30kDa

Applications

Reactivity	Oryza sativa
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Leaves, Stems, Spikes, Milk Grain
Cellular location
Customer validation	WB(Oryza sativa)