제품 > 항체 > 다중클론항체(pAb)

GF14f Rabbit pAb (A20626)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - GF14f Rabbit pAb (A20626)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GF14f Rabbit pAb (A20626) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

Overview

Product nameGF14f Rabbit pAb
Catalog No.A20626
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Is associated with a DNA binding complex that binds to the G box, a well-characterized cis-acting DNA regulatory element found in plant genes.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of Oryza sativa GF14f. (Q06967).
SequenceMSPAEASREENVYMAKLAEQAERYEEMVEFMEKVAKTTDVGELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEESRGNEAYVASIKEYRSRIETELS
Gene ID
Swiss Prot
SynonymsGF14F; OsGF14a; SR14-3-3; OsJ_12311; GF14f
Calculated MW29kDa
Observed MW29kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSpikes
Cellular locationcytoplasm, nucleus
    ABclonal:Western blot - GF14f Rabbit pAb (A20626)}

    Western blot - GF14f Rabbit pAb (A20626)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using GF14f Rabbit pAb (A20626) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)