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GFM1 Rabbit pAb (A5077)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GFM1 Rabbit pAb (A5077)

Western blot analysis of various lysates, using GFM1 Rabbit pAb (A5077) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.

ABclonal:Immunohistochemistry - GFM1 Rabbit pAb (A5077)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GFM1 Rabbit pAb (A5077) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GFM1 Rabbit pAb (A5077)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using GFM1 Rabbit pAb (A5077) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameGFM1 Rabbit pAb
Catalog No.A5077
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Eukaryotes contain two protein translational systems, one in the cytoplasm and one in the mitochondria. Mitochondrial translation is crucial for maintaining mitochondrial function and mutations in this system lead to a breakdown in the respiratory chain-oxidative phosphorylation system and to impaired maintenance of mitochondrial DNA. This gene encodes one of the mitochondrial translation elongation factors. Its role in the regulation of normal mitochondrial function and in different disease states attributed to mitochondrial dysfunction is not known.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 482-751 of human GFM1 (NP_079272.4).
SequenceVYFDTENKETVISGMGELHLEIYAQRLEREYGCPCITGKPKVAFRETITAPVPFDFTHKKQSGGAGQYGKVIGVLEPLDPEDYTKLEFSDETFGSNIPKQFVPAVEKGFLDACEKGPLSGHKLSGLRFVLQDGAHHMVDSNEISFIRAGEGALKQALANATLCILEPIMAVEVVAPNEFQGQVIAGINRRHGVITGQDGVEDYFTLYADVPLNDMFGYSTELRSCTEGKGEYTMEYSRYQPCLPSTQEDVINKYLEATGQLPVKKGKAKN
Gene ID
Swiss Prot
SynonymsEFG; GFM; EFG1; EFGM; EGF1; hEFG1; COXPD1; mtEF-G1; GFM1
Calculated MW83kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse kidney, Rat kidney
Cellular locationMitochondrion

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ABclonal:Western blot - GFM1 Rabbit pAb (A5077)}

Western blot - GFM1 Rabbit pAb (A5077)

Western blot analysis of various lysates, using GFM1 Rabbit pAb (A5077) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 45s.
ABclonal:Immunohistochemistry - GFM1 Rabbit pAb (A5077)}

Immunohistochemistry - GFM1 Rabbit pAb (A5077)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using GFM1 Rabbit pAb (A5077) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GFM1 Rabbit pAb (A5077)}

Immunohistochemistry - GFM1 Rabbit pAb (A5077)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using GFM1 Rabbit pAb (A5077) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)