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GH1 Rabbit pAb (A7675)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GH1 Rabbit pAb (A7675)

Western blot analysis of lysates from Mouse liver using GH1 Rabbit pAb(A7675) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:10s.

ABclonal:Immunohistochemistry - GH1 Rabbit pAb (A7675)

Immunohistochemistry analysis of paraffin-embedded Human placenta using GH1 Rabbit pAb (A7675) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GH1 Rabbit pAb (A7675)

Immunofluorescence analysis of paraffin-embedded human placenta using GH1 Rabbit pAb (A7675) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGH1 Rabbit pAb
Catalog No.A7675
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human GH1 (NP_000506.2).
SequenceELLRISLLLIQSWLEPVQFLRSVFANSLVYGASDSNVYDLLKDLEEGIQTLMGRLEDGSPRTGQIFKQTYSKFDTNSHNDDALLKNYGLLYCFRKDMDKVE
Gene ID
Swiss Prot
SynonymsGH; GHN; GH-N; GHB5; IGHD2; hGH-N; IGHD1A; IGHD1B; GH1
Calculated MW25kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationSecreted

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ABclonal:Western blot - GH1 Rabbit pAb (A7675)}

Western blot - GH1 Rabbit pAb (A7675)

Western blot analysis of lysates from Mouse liver using GH1 Rabbit pAb(A7675) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:10s.
ABclonal:Immunohistochemistry - GH1 Rabbit pAb (A7675)}

Immunohistochemistry - GH1 Rabbit pAb (A7675)

Immunohistochemistry analysis of paraffin-embedded Human placenta using GH1 Rabbit pAb (A7675) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GH1 Rabbit pAb (A7675)}

Immunofluorescence - GH1 Rabbit pAb (A7675)

Immunofluorescence analysis of paraffin-embedded human placenta using GH1 Rabbit pAb (A7675) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

재조합 단백질 (1)

Secondary Antibodies (26)