제품 > 항체 > 다중클론항체(pAb)

GHRL Rabbit pAb (A12581)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - GHRL Rabbit pAb (A12581)

Western blot analysis of lysates from rat liver, using GHRL Rabbit pAb (A12581) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GHRL Rabbit pAb (A12581)

Immunohistochemistry analysis of paraffin-embedded human stomach using GHRL Rabbit pAb (A12581) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GHRL Rabbit pAb (A12581)

Immunohistochemistry analysis of paraffin-embedded human rectum using GHRL Rabbit pAb (A12581) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GHRL Rabbit pAb (A12581)

Immunohistochemistry analysis of paraffin-embedded human rectal cancer using GHRL Rabbit pAb (A12581) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameGHRL Rabbit pAb
Catalog No.A12581
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GHRL (NP_057446.1).
SequenceMPSPGTVCSLLLLGMLWLDLAMAGSSFLSPEHQRVQQRKESKKPPAKLQPRALAGWLRPEDGGQAEGAEDELEVRFNAPFDVGIKLSGVQYQQHSQALGKFLQDILWEEAKEAPADK
Gene ID
Swiss Prot
SynonymsMTLRP; GHRL
Calculated MW13kDa
Observed MW15kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesRat liver
Cellular locationSecreted
Customer validation

WB(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - GHRL Rabbit pAb (A12581)}

Western blot - GHRL Rabbit pAb (A12581)

Western blot analysis of lysates from rat liver, using GHRL Rabbit pAb (A12581) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GHRL Rabbit pAb (A12581)}

Immunohistochemistry - GHRL Rabbit pAb (A12581)

Immunohistochemistry analysis of paraffin-embedded human stomach using GHRL Rabbit pAb (A12581) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GHRL Rabbit pAb (A12581)}

Immunohistochemistry - GHRL Rabbit pAb (A12581)

Immunohistochemistry analysis of paraffin-embedded human rectum using GHRL Rabbit pAb (A12581) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GHRL Rabbit pAb (A12581)}

Immunohistochemistry - GHRL Rabbit pAb (A12581)

Immunohistochemistry analysis of paraffin-embedded human rectal cancer using GHRL Rabbit pAb (A12581) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A12581? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

항체 (1)

제품 (1)

재조합 단백질 (1)

Secondary Antibodies (26)