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GIF Rabbit pAb (A6914)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - GIF Rabbit pAb (A6914)

Western blot analysis of extracts of various cell lines, using GIF antibody (A6914) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - GIF Rabbit pAb (A6914)

Immunofluorescence analysis of A549 cells using GIF antibody (A6914). Blue: DAPI for nuclear staining.

Overview

Product nameGIF Rabbit pAb
Catalog No.A6914
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GIF (NP_005133.2).
SequenceTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLKAQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAILALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKISMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTYLDVPQVTCSPDHEVQPTLPS
Gene ID
Swiss Prot
SynonymsIF; GIF; INF; IFMH; TCN3
Calculated MW45kDa
Observed MW45kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse pancreas, Mouse heart
Cellular locationSecreted

Documents

Certificate of Compliance

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    ABclonal:Western blot - GIF Rabbit pAb (A6914)}

    Western blot - GIF Rabbit pAb (A6914)

    Western blot analysis of extracts of various cell lines, using GIF antibody (A6914) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunofluorescence - GIF Rabbit pAb (A6914)}

    Immunofluorescence - GIF Rabbit pAb (A6914)

    Immunofluorescence analysis of A549 cells using GIF antibody (A6914). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (25)