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GLI2 Rabbit pAb (A16864)

Publications (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GLI2 Rabbit pAb (A16864)

Western blot analysis of various lysates using GLI2 Rabbit pAb (A16864) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - GLI2 Rabbit pAb (A16864)

Immunofluorescence analysis of L-929 cells using GLI2 Rabbit pAb (A16864) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GLI2 Rabbit pAb (A16864)

Immunofluorescence analysis of U-2 OS cells using GLI2 Rabbit pAb (A16864) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GLI2 Rabbit pAb (A16864)

Immunofluorescence analysis of U-2 OS cells using GLI2 Rabbit pAb (A16864) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGLI2 Rabbit pAb
Catalog No.A16864
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and promoter-specific chromatin binding activity. Acts upstream of or within several processes, including animal organ development; chordate embryonic development; and regulation of smoothened signaling pathway. Located in several cellular components, including axoneme; ciliary tip; and nuclear speck. Is expressed in several structures, including central nervous system; embryo mesenchyme; eye; genitourinary system; and jaw. Human ortholog(s) of this gene implicated in Culler-Jones syndrome; holoprosencephaly 9; and spina bifida. Orthologous to human GLI2 (GLI family zinc finger 2).
ImmunogenRecombinant protein of mouse GLI2.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsGLI2; CJS; HPE9; PHS2; THP1; THP2; Gli2
Calculated MW165kDa
Observed MW170kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, MCF7, HeLa, NIH/3T3
Cellular locationCytoplasm, Nucleus.
Customer validation

WB(Mus musculus)

Documents

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    ABclonal:Western blot - GLI2 Rabbit pAb (A16864)}

    Western blot - GLI2 Rabbit pAb (A16864)

    Western blot analysis of various lysates using GLI2 Rabbit pAb (A16864) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - GLI2 Rabbit pAb (A16864)}

    Immunofluorescence - GLI2 Rabbit pAb (A16864)

    Immunofluorescence analysis of L-929 cells using GLI2 Rabbit pAb (A16864) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GLI2 Rabbit pAb (A16864)}

    Immunofluorescence - GLI2 Rabbit pAb (A16864)

    Immunofluorescence analysis of U-2 OS cells using GLI2 Rabbit pAb (A16864) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GLI2 Rabbit pAb (A16864)}

    Immunofluorescence - GLI2 Rabbit pAb (A16864)

    Immunofluorescence analysis of U-2 OS cells using GLI2 Rabbit pAb (A16864) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16864? Please let us know so that we can cite the reference in this datasheet.