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GNAI3 Rabbit pAb (A15674)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GNAI3 Rabbit pAb (A15674)

Western blot analysis of various lysates using GNAI3 Rabbit pAb (A15674) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.

ABclonal:Western blot - GNAI3 Rabbit pAb (A15674)

Western blot analysis of various lysates using GNAI3 Rabbit pAb (A15674) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameGNAI3 Rabbit pAb
Catalog No.A15674
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAI3 (NP_006487.1).
SequenceMGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGEAAR
Gene ID
Swiss Prot
Synonyms87U6; HG1A; ARCND1; ARCODS; GNAI3
Calculated MW41kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesLO2, HepG2, Mouse kidney, Mouse brain, Rat kidney
Cellular locationCell membrane, Cytoplasm, Lipid-anchor, Membrane, centrosome, cytoskeleton, microtubule organizing center.

Documents

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ABclonal:Western blot - GNAI3 Rabbit pAb (A15674)}

Western blot - GNAI3 Rabbit pAb (A15674)

Western blot analysis of various lysates using GNAI3 Rabbit pAb (A15674) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 30s.
ABclonal:Western blot - GNAI3 Rabbit pAb (A15674)}

Western blot - GNAI3 Rabbit pAb (A15674)

Western blot analysis of various lysates using GNAI3 Rabbit pAb (A15674) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

* For research use only. Not for therapeutic or diagnostic purposes.

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