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GNAT1 Rabbit pAb (A6496)

Reviews (2)Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GNAT1 Rabbit pAb (A6496)

Western blot analysis of various lysates, using GNAT1 Rabbit pAb (A6496) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for GNAT1 Rabbit pAb(A6496)
Review
ABclonal: review for GNAT1 Rabbit pAb(A6496)

Overview

Product nameGNAT1 Rabbit pAb
Catalog No.A6496
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 58-178 of human GNAT1 (NP_000163.2).
SequenceSLEECLEFIAIIYGNTLQSILAIVRAMTTLNIQYGDSARQDDARKLMHMADTIEEGTMPKEMSDIIQRLWKDSGIQACFERASEYQLNDSAGYYLSDLERLVTPGYVPTEQDVLRSRVKTT
Gene ID
Swiss Prot
SynonymsGBT1; HG1F; GNATR; CSNB1G; CSNBAD3; GNAT1
Calculated MW40kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse eye, Rat eye
Cellular locationapical plasma membrane, cytosol, photoreceptor outer segment membrane, plasma membrane.
Customer validation

(Zebrafish)

WB(Danio rerio)

IF(Danio rerio)

Documents

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    ABclonal:Western blot - GNAT1 Rabbit pAb (A6496)}

    Western blot - GNAT1 Rabbit pAb (A6496)

    Western blot analysis of various lysates, using GNAT1 Rabbit pAb (A6496) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)