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GOLPH4 Rabbit mAb (A19790)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - GOLPH4 Rabbit mAb (A19790)

Western blot analysis of extracts of various cell lines, using GOLPH4 antibody (A19790) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameGOLPH4 Rabbit mAb
Catalog No.A19790
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2315
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GOLPH4 (O00461).
SequenceMGNGMCSRKQKRIFQTLLLLTVVFGFLYGAMLYYELQTQLRKAEAVALKYQQHQESLSAQLQVVYEHRSRLEKSLQKERLEHKKAKEDFLVYKLEAQETL
Gene ID
Swiss Prot
SynonymsP138; GIMPC; GOLPH4; GPP130
Calculated MW82kDa
Observed MW130kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, Hep G2
Cellular locationCis-Golgi network, Golgi apparatus, Golgi lumen, Transport vesicle

Documents

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    ABclonal:Western blot - GOLPH4 Rabbit mAb (A19790)}

    Western blot - GOLPH4 Rabbit mAb (A19790)

    Western blot analysis of extracts of various cell lines, using GOLPH4 antibody (A19790) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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