제품 > 항체 > 다중클론항체(pAb)

GPT2 Rabbit pAb (A23670)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - GPT2 Rabbit pAb (A23670)

Western blot analysis of lysates from HepG2 cells, using GPT2 Rabbit pAb (A23670) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameGPT2 Rabbit pAb
Catalog No.A23670
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a mitochondrial alanine transaminase, a pyridoxal enzyme that catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate. Alanine transaminases play roles in gluconeogenesis and amino acid metabolism in many tissues including skeletal muscle, kidney, and liver. Activating transcription factor 4 upregulates this gene under metabolic stress conditions in hepatocyte cell lines. A loss of function mutation in this gene has been associated with developmental encephalopathy. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 310-410 of human GPT2. (NP_597700.1).
SequenceDCRFHSFKKVLYEMGPEYSSNVELASFHSTSKGYMGECGYRGGYMEVINLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVL
Gene ID
Swiss Prot
SynonymsALT2; GPT 2; MRT49; NEDSPM; GPT2
Calculated MW58kDa
Observed MW58 kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2
Cellular locationmitochondrial matrix
Customer validation

WB(Mus musculus)

Documents

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ABclonal:Western blot - GPT2 Rabbit pAb (A23670)}

Western blot - GPT2 Rabbit pAb (A23670)

Western blot analysis of lysates from HepG2 cells, using GPT2 Rabbit pAb (A23670) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A23670? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)