제품 > 항체 > 다중클론항체(pAb)

GRB10 Rabbit pAb (A0634)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - GRB10 Rabbit pAb (A0634)

Western blot analysis of extracts of HeLa cells, using GRB10 antibody (A0634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameGRB10 Rabbit pAb
Catalog No.A0634
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GRB10 (NP_005302.3).
SequenceMALAGCPDSFLHHPYYQDKVEQTPRSQQDPAGPGLPAQSDRLANHQEDDVDLEALVNDMNASLESLYSACSMQSDTVPLLQNGQHARSQPRASGPPRSIQ
Gene ID
Swiss Prot
SynonymsRSS; IRBP; MEG1; GRB-IR; Grb-10
Calculated MW67kDa
Observed MW70kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa
Cellular locationCytoplasm
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

ABclonal:Western blot - GRB10 Rabbit pAb (A0634)}

Western blot - GRB10 Rabbit pAb (A0634)

Western blot analysis of extracts of HeLa cells, using GRB10 antibody (A0634) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0634? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)