제품 > 항체 > 다중클론항체(pAb)

GRID2 Rabbit pAb (A19314)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GRID2 Rabbit pAb (A19314)

Western blot analysis of lysates from Mouse brian, using GRID2 Rabbit pAb (A19314) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GRID2 Rabbit pAb (A19314)

Immunofluorescence analysis of C6 cells using GRID2 Rabbit pAb (A19314) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GRID2 Rabbit pAb (A19314)

Immunofluorescence analysis of L929 cells using GRID2 Rabbit pAb (A19314) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GRID2 Rabbit pAb (A19314)

Immunofluorescence analysis of U-2 OS cells using GRID2 Rabbit pAb (A19314) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGRID2 Rabbit pAb
Catalog No.A19314
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 900-1007 of human GRID2 (NP_001501.2).
SequenceIDLTPLDIDTLPTRQALEQISDFRNTHITTTTFIPEQIQTLSRTLSAKAASGFTFGNVPEHRTGPFRHRAPNGGFFRSPIKTMSSIPYQPTPTLGLNLGNDPDRGTSI
Gene ID
Swiss Prot
SynonymsGluD2; SCAR18; GRID2
Calculated MW113kDa
Observed MW113kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse brian
Cellular locationdendritic spine, glutamatergic synapse, parallel fiber to Purkinje cell synapse, plasma membrane, postsynaptic density membrane, postsynaptic membrane, synapse

Documents

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    ABclonal:Western blot - GRID2 Rabbit pAb (A19314)}

    Western blot - GRID2 Rabbit pAb (A19314)

    Western blot analysis of lysates from Mouse brian, using GRID2 Rabbit pAb (A19314) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - GRID2 Rabbit pAb (A19314)}

    Immunofluorescence - GRID2 Rabbit pAb (A19314)

    Immunofluorescence analysis of C6 cells using GRID2 Rabbit pAb (A19314) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GRID2 Rabbit pAb (A19314)}

    Immunofluorescence - GRID2 Rabbit pAb (A19314)

    Immunofluorescence analysis of L929 cells using GRID2 Rabbit pAb (A19314) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - GRID2 Rabbit pAb (A19314)}

    Immunofluorescence - GRID2 Rabbit pAb (A19314)

    Immunofluorescence analysis of U-2 OS cells using GRID2 Rabbit pAb (A19314) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)