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GRIK2 Rabbit mAb (A9722)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GRIK2 Rabbit mAb (A9722)

Western blot analysis of extracts of Mouse brain, using GRIK2 Rabbit mAb (A9722) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - GRIK2 Rabbit mAb (A9722)

Western blot analysis of extracts of 293T cells, using GRIK2 Rabbit mAb (A9722) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded human colon carcinoma tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded human kidney tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded mouse intestin tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded rat kidney tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameGRIK2 Rabbit mAb
Catalog No.A9722
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1718
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing at multiple sites within the first and second transmembrane domains, which is thought to alter the structure and function of the receptor complex. Alternatively spliced transcript variants encoding different isoforms have also been described for this gene. Mutations in this gene have been associated with autosomal recessive cognitive disability.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 50-150 of human GRIK2 (Q13002).
SequenceMGAEELAFRFAVNTINRNRTLLPNTTLTYDTQKINLYDSFEASKKACDQLSLGVAAIFGPSHSSSANAVQSICNALGVPHIQTRWKHQVSDNKDSFYVSLY
Gene ID
Swiss Prot
SynonymsEAA4; GLR6; MRT6; GLUK6; GLUR6; GluK2; NEDLAS
Calculated MW103kDa
Observed MW100kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, 293T
Cellular locationCell junction, Cell membrane, Multi-pass membrane protein, Postsynaptic cell membrane, Synapse

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ABclonal:Western blot - GRIK2 Rabbit mAb (A9722)}

Western blot - GRIK2 Rabbit mAb (A9722)

Western blot analysis of extracts of Mouse brain, using GRIK2 Rabbit mAb (A9722) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - GRIK2 Rabbit mAb (A9722)}

Western blot - GRIK2 Rabbit mAb (A9722)

Western blot analysis of extracts of 293T cells, using GRIK2 Rabbit mAb (A9722) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)}

Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded human colon carcinoma tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)}

Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded human kidney tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)}

Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded mouse intestin tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - GRIK2 Rabbit mAb (A9722)}

Immunohistochemistry - GRIK2 Rabbit mAb (A9722)

Immunohistochemistry analysis of GRIK2 in paraffin-embedded rat kidney tissue using GRIK2 Rabbit mAb (A9722) at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)