제품 > 항체 > 다중클론항체(pAb)

GRPEL1 Rabbit pAb (A4999)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GRPEL1 Rabbit pAb (A4999)

Western blot analysis of lysates from Mouse liver, using GRPEL1 Rabbit pAb (A4999) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - GRPEL1 Rabbit pAb (A4999)

Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human liver tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GRPEL1 Rabbit pAb (A4999)

Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human tonsil tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GRPEL1 Rabbit pAb (A4999)

Immunofluorescence analysis of HepG2 cells using GRPEL1 Rabbit pAb (A4999) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGRPEL1 Rabbit pAb
Catalog No.A4999
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables identical protein binding activity and unfolded protein binding activity. Predicted to be involved in protein import into mitochondrial matrix. Located in mitochondrial matrix and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-217 of human GRPEL1 (NP_079472.1).
SequenceMAAQCVRLARRSLPALALSLRPSPRLLCTATKQKNSGQNLEEDMGQSEQKADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAKLYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQIQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPALVGVVKEA
Gene ID
Swiss Prot
SynonymsGrpE; HMGE; mt-GrpE#1; GRPEL1
Calculated MW24kDa
Observed MW24kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse liver
Cellular locationMitochondrion matrix

Documents

Certificate of Compliance

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    ABclonal:Western blot - GRPEL1 Rabbit pAb (A4999)}

    Western blot - GRPEL1 Rabbit pAb (A4999)

    Western blot analysis of lysates from Mouse liver, using GRPEL1 Rabbit pAb (A4999) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - GRPEL1 Rabbit pAb (A4999)}

    Immunohistochemistry - GRPEL1 Rabbit pAb (A4999)

    Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human liver tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GRPEL1 Rabbit pAb (A4999)}

    Immunohistochemistry - GRPEL1 Rabbit pAb (A4999)

    Immunohistochemistry analysis of GRPEL1 in paraffin-embedded Human tonsil tissue using GRPEL1 Rabbit pAb (A4999) at a dilution of  1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - GRPEL1 Rabbit pAb (A4999)}

    Immunofluorescence - GRPEL1 Rabbit pAb (A4999)

    Immunofluorescence analysis of HepG2 cells using GRPEL1 Rabbit pAb (A4999) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)