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GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from THP-1 cells, using GSDMD (Full Length+N terminal) Rabbit pAb (A10164) at 1:400 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and 293T transfected with GSDMD-N using GSDMD (Full Length+N terminal) (A10164) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from wild type (WT) and 293T transfected with GSDMD using GSDMD (Full Length+N terminal) (A10164) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunofluorescence - GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Immunofluorescence analysis of HeLa cells using GSDMD (Full Length+N terminal) Rabbit pAb (A10164) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using GSDMD (Full Length+N terminal) Rabbit pAb (A10164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using GSDMD (Full Length+N terminal) Rabbit pAb (A10164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using GSDMD (Full Length+N terminal) Rabbit pAb (A10164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-3 cells using GSDMD (Full Length+N terminal) Rabbit pAb (A10164) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	GSDMD (Full length+N terminal) Rabbit pAb
Catalog No.	A10164
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables phospholipid binding activity and wide pore channel activity. Involved in several processes, including defense response to bacterium; positive regulation of interleukin-1 beta production; and protein-containing complex assembly. Acts upstream of or within cellular response to extracellular stimulus. Located in cytosol; extracellular space; and plasma membrane. Part of NLRP3 inflammasome complex. Is expressed in several structures, including gut; liver; lung; metanephros; and spleen. Orthologous to human GSDMD (gasdermin D).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 80-163 of mouse Cleaved GSDMD (NP_081236.1).
Sequence	SFKVSDVVDGNIQGRVMLSGMGEGKISGGAAVSDSSSASMNVCILRVTQKTWETMQHERHLQQPENKILQQLRSRGDDLFVVTE
Gene ID
Swiss Prot
Synonyms	DF5L; M2-4; Dfna5l; GsdmD-1; Gsdmdc1; 1810036L03Rik; GSDMD (Full Length+N terminal)
Calculated MW	53kDa
Observed MW	29kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	THP-1 treated by TPA and LPS, 293T transfected with GSDMD-N, 293T transfected with GSDMD
Cellular location	cytosol, extracellular region, extracellular space, NLRP3 inflammasome complex, nucleoplasm, plasma membrane
Customer validation	(Human chronic myeloid leukemia cells, HeLa cells, N9) WB(Homo sapiens,Rattus norvegicus, Mus musculus, Homo sapiens, Rattus norvegicus) IHC(Mus musculus) IF(Homo sapiens, Rattus norvegicus, Mus musculus) IF(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증Inflammasome Signaling Pathway

GSDMD (Full length+N terminal) Rabbit pAb images

Western blot - GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Immunofluorescence - GSDMD (Full length+N terminal) Rabbit pAb (A10164)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Biochemical and Biophysical Research Communications

Application:

IF:

2.5

Species:

Homo sapiens,Rattus norvegicus

PMID:

31718799

Title:

The role of microglia mediated pyroptosis in neonatal hypoxic-ischemic brain damage

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Molecular Neurodegeneration

Application:

IF:

15.1

Species:

Mus musculus

PMID:

32295623

Title:

NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Nutrients

Application:

IF:

5.9

Species:

Mus musculus

PMID:

32456088

Title:

Punicalagin Protects Diabetic Nephropathy by Inhibiting Pyroptosis Based on TXNIP/NLRP3 Pathway

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Cells

Application:

IHC

IF:

7.7

Species:

Mus musculus

PMID:

32842536

Title:

Loganin Attenuates High Glucose-Induced Schwann Cells Pyroptosis by Inhibiting ROS Generation and NLRP3 Inflammasome Activation

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

J Neuroinflammation

Application:

IF:

9.6

Species:

Rattus norvegicus

PMID:

34666787

Title:

Blockade of the NLRP3/caspase-1 axis attenuates ketamine-induced hippocampus pyroptosis and cognitive impairment in neonatal rats

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Journal of cellular and molecular medicine

Application:

IF:

5.3

Species:

Rattus norvegicus

PMID:

35775127

Title:

Activation of adenosine A3 receptor attenuates progression of osteoarthritis through inhibiting the NLRP3/caspase-1/GSDMD induced signalling - PMC

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Application:

IF:

3.9

Species:

Mus musculus

PMID:

35985369

Title:

Copper induces microglia-mediated neuroinflammation through ROS/NF-κB pathway and mitophagy disorder

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Respiratory research

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

36527086

Title:

Disulfiram attenuates hypoxia-induced pulmonary hypertension by inhibiting GSDMD cleavage and pyroptosis in HPASMCs - PMC

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Life sciences

Application:

IF:

5.2

Species:

Rattus norvegicus

PMID:

37120013

Title:

Anti-inflammatory activity of carvacrol protects the heart from lipopolysaccharide-induced cardiac dysfunction by inhibiting pyroptosis via NLRP3/Caspase1/Gasdermin …

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

4.8

Species:

Mus musculus

PMID:

37651853

Title:

3, 4-Benzopyrene (Bap) aggravated abdominal aortic aneurysm formation by targeting pyroptosis in smooth muscle cells through ET-1 mediated NLRP3

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

International journal of molecular sciences

Application:

WB, IF

IF:

5.6

Species:

Homo sapiens, Rattus norvegicus

PMID:

36902422

Title:

Dual Role of Mitogen-Activated Protein Kinase 8 Interacting Protein-1 in Inflammasome and Pancreatic -Cell Function - PMC

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

WB, IF

IF:

9.6

Species:

Mus musculus

PMID:

36171629

Title:

Erbin protects against sepsis-associated encephalopathy by attenuating microglia pyroptosis via IRE1/Xbp1s-Ca2+ axis - PMC

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.6

Species:

Mus musculus

PMID:

38037161

Title:

TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy - PMC

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Life sciences

Application:

IF:

5.2

Species:

Mus musculus

PMID:

37467885

Title:

Targeting NAT10 protects against sepsis-induced skeletal muscle atrophy by inhibiting ROS/NLRP3

References for GSDMD (Full length+N terminal) Rabbit pAb (A10164)

Product:

GSDMD (Full length+N terminal) Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.7

Species:

Homo sapiens

PMID:

39531828

Title:

Deficiency of muscle-generated brain-derived neurotrophic factor causes inflammatory myopathy through reactive oxygen species-mediated necroptosis and pyroptosis - PMC