제품 > 항체 > 단일클론항체(mAb)

GSK-3α/β Rabbit mAb (A22666)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GSK-3α/β Rabbit mAb (A22666)

Western blot analysis of various lysates, using GSK-3α/β Rabbit mAb (A22666) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded mouse colon tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded human liver tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded mouse kidney tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded rat colon tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded mouse testis tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)

Confocal imaging of HeLa cells using GSK-3α/β Rabbit mAb (A22666, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)

Confocal imaging of paraffin-embedded Mouse brain tissue using GSK-3α/β Rabbit mAb (A22666, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)

Confocal imaging of paraffin-embedded Rat brain tissue using GSK-3α/β Rabbit mAb (A22666, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameGSK-3α/β Rabbit mAb
Catalog No.A22666
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55355
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant protein of human GSK-3α/β.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsGSK3B; gsk-3β; GSK-3α/β
Calculated MW46kDa/48kDa
Observed MWRefer to figures
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:60000
  • IHC-P 1:500- 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationCell membrane, Cytoplasm, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

    ABclonal:Western blot - GSK-3α/β Rabbit mAb (A22666)}

    Western blot - GSK-3α/β Rabbit mAb (A22666)

    Western blot analysis of various lysates, using GSK-3α/β Rabbit mAb (A22666) at 1:50000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)}

    Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

    Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded mouse colon tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)}

    Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

    Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded human liver tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)}

    Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

    Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded mouse kidney tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)}

    Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

    Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded rat colon tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)}

    Immunohistochemistry - GSK-3α/β Rabbit mAb (A22666)

    Immunohistochemistry analysis of GSK-3α/β in paraffin-embedded mouse testis tissue using GSK-3α/β Rabbit mAb (A22666) at a dilution of 1:800 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)}

    Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)

    Confocal imaging of HeLa cells using GSK-3α/β Rabbit mAb (A22666, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)}

    Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)

    Confocal imaging of paraffin-embedded Mouse brain tissue using GSK-3α/β Rabbit mAb (A22666, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
    ABclonal:Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)}

    Immunofluorescence - GSK-3α/β Rabbit mAb (A22666)

    Confocal imaging of paraffin-embedded Rat brain tissue using GSK-3α/β Rabbit mAb (A22666, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.

    * For research use only. Not for therapeutic or diagnostic purposes.

    항체 (2)

    Secondary Antibodies (26)