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GTF2B Rabbit pAb (A1708)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GTF2B Rabbit pAb (A1708)

Western blot analysis of various lysates using GTF2B Rabbit pAb (A1708) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded human colon using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded human esophageal using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded mouse testis using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GTF2B Rabbit pAb (A1708)

Immunofluorescence analysis of L929 cells using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGTF2B Rabbit pAb
Catalog No.A1708
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-154 of GTF2B (NP_001505.1).
SequenceVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGR
Gene ID
Swiss Prot
SynonymsTF2B; TFIIB; GTF2B
Calculated MW35kDa
Observed MW32kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, Mouse thymus, Rat testis
Cellular locationNucleus
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - GTF2B Rabbit pAb (A1708)}

Western blot - GTF2B Rabbit pAb (A1708)

Western blot analysis of various lysates using GTF2B Rabbit pAb (A1708) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)}

Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)}

Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded human colon using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)}

Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded human esophageal using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GTF2B Rabbit pAb (A1708)}

Immunohistochemistry - GTF2B Rabbit pAb (A1708)

Immunohistochemistry analysis of paraffin-embedded mouse testis using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GTF2B Rabbit pAb (A1708)}

Immunofluorescence - GTF2B Rabbit pAb (A1708)

Immunofluorescence analysis of L929 cells using GTF2B Rabbit pAb (A1708) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1708? Please let us know so that we can cite the reference in this datasheet.

Secondary Antibodies (26)