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GTF2F2 Rabbit mAb (A0536)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GTF2F2 Rabbit mAb (A0536)

Western blot analysis of various lysates using GTF2F2 Rabbit mAb (A0536) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

Immunohistochemistry analysis of GTF2F2 in paraffin-embedded human colon tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

Immunohistochemistry analysis of GTF2F2 in paraffin-embedded rat colon tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

Immunohistochemistry analysis of GTF2F2 in paraffin-embedded human lung tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

Immunohistochemistry analysis of GTF2F2 in paraffin-embedded human esophagus tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

Immunohistochemistry analysis of GTF2F2 in paraffin-embedded mouse testis tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Overview

Product nameGTF2F2 Rabbit mAb
Catalog No.A0536
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2513
Predicted to enable RNA polymerase II general transcription initiation factor activity. Involved in transcription by RNA polymerase II. Located in microtubule cytoskeleton and nucleoplasm. Part of transcription preinitiation complex.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GTF2F2 (P13984).
SequenceMAERGELDLTGAKQNTGVWLVKVPKYLSQQWAKASGRGEVGKLRIAKTQGRTEVSFTLNEDLANIHDIGGKPASVSAPREHPFVLQSVGGQTLTVFTESS
Gene ID
Swiss Prot
SynonymsBTF4; RAP30; TF2F2; TFIIF; GTF2F2
Calculated MW28kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, HepG2, NIH/3T3, Mouse liver, Mouse testis, Rat testis
Cellular locationNucleus

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - GTF2F2 Rabbit mAb (A0536)}

    Western blot - GTF2F2 Rabbit mAb (A0536)

    Western blot analysis of various lysates using GTF2F2 Rabbit mAb (A0536) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 1s.
    ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)}

    Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

    Immunohistochemistry analysis of GTF2F2 in paraffin-embedded human colon tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)}

    Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

    Immunohistochemistry analysis of GTF2F2 in paraffin-embedded rat colon tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)}

    Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

    Immunohistochemistry analysis of GTF2F2 in paraffin-embedded human lung tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)}

    Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

    Immunohistochemistry analysis of GTF2F2 in paraffin-embedded human esophagus tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)}

    Immunohistochemistry - GTF2F2 Rabbit mAb (A0536)

    Immunohistochemistry analysis of GTF2F2 in paraffin-embedded mouse testis tissue using GTF2F2 Rabbit mAb (A0536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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