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GTPBP4 Rabbit pAb (A4565)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - GTPBP4 Rabbit pAb (A4565)

Western blot analysis of various lysates using GTPBP4 Rabbit pAb (A4565) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameGTPBP4 Rabbit pAb
Catalog No.A4565
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 385-634 of human GTPBP4 (NP_036473.2).
SequenceRKRMETEESRKKRERDLELEMGDDYILDLQKYWDLMNLSEKHDKIPEIWEGHNIADYIDPAIMKKLEELEKEEELRTAAGEYDSVSESEDEEMLEIRQLAKQIREKKKLKILESKEKNTQGPRMPRTAKKVQRTVLEKEMRSLGVDMDDKDDAHYAVQARRSRSITRKRKREDSAPPSSVARSGSCSRTPRDVSGLRDVKMVKKAKTMMKNAQKKMNRLGKKGEADRHVFDMKPKHLLSGKRKAGKKDRR
Gene ID
Swiss Prot
SynonymsNGB; CRFG; NOG1; GTPBP4
Calculated MW74kDa
Observed MW74kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, HeLa, Jurkat, MCF7, HT-1080, K-562
Cellular locationNucleus, nucleolus
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - GTPBP4 Rabbit pAb (A4565)}

Western blot - GTPBP4 Rabbit pAb (A4565)

Western blot analysis of various lysates using GTPBP4 Rabbit pAb (A4565) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)}

Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)}

Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Human esophageal using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)}

Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)}

Immunohistochemistry - GTPBP4 Rabbit pAb (A4565)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using GTPBP4 Rabbit pAb (A4565) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)