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GTPBP4 Rabbit mAb (A4743)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - GTPBP4 Rabbit mAb (A4743)

Western blot analysis of various lysates using GTPBP4 Rabbit mAb (A4743) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GTPBP4 Rabbit mAb (A4743)

Immunohistochemistry analysis of paraffin-embedded Human colon using GTPBP4 Rabbit mAb (A4743) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameGTPBP4 Rabbit mAb
Catalog No.A4743
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1115
GTP-binding proteins are GTPases and function as molecular switches that can flip between two states: active, when GTP is bound, and inactive, when GDP is bound. 'Active' in this context usually means that the molecule acts as a signal to trigger other events in the cell. When an extracellular ligand binds to a G-protein-linked receptor, the receptor changes its conformation and switches on the trimeric G proteins that associate with it by causing them to eject their GDP and replace it with GTP. The switch is turned off when the G protein hydrolyzes its own bound GTP, converting it back to GDP. But before that occurs, the active protein has an opportunity to diffuse away from the receptor and deliver its message for a prolonged period to its downstream target.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2-92 of human GTPBP4 (Q9BZE4).
SequenceAHYNFKKITVVPSAKDFIDLTLSKTQRKTPTVIHKHYQIHRIRHFYMRKVKFTQQNYHDRLSQILTDFPKLDDIHPFYADLMNILYDKDHY
Gene ID
Swiss Prot
SynonymsNGB; CRFG; NOG1; GTPBP4
Calculated MW74kDa
Observed MW74kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, Jurkat
Cellular locationNucleus, Nucleolus

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ABclonal:Western blot - GTPBP4 Rabbit mAb (A4743)}

Western blot - GTPBP4 Rabbit mAb (A4743)

Western blot analysis of various lysates using GTPBP4 Rabbit mAb (A4743) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GTPBP4 Rabbit mAb (A4743)}

Immunohistochemistry - GTPBP4 Rabbit mAb (A4743)

Immunohistochemistry analysis of paraffin-embedded Human colon using GTPBP4 Rabbit mAb (A4743) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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