제품 > 항체 > 다중클론항체(pAb)

Gelsolin Rabbit pAb (A16869)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Gelsolin Rabbit pAb (A16869)

Western blot analysis of various lysates using Gelsolin Rabbit pAb (A16869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - Gelsolin Rabbit pAb (A16869)

Western blot analysis of various lysates using Gelsolin Rabbit pAb (A16869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Gelsolin Rabbit pAb (A16869)

Immunofluorescence analysis of C6 cells using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Gelsolin Rabbit pAb (A16869)

Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Gelsolin Rabbit pAb (A16869)

Immunofluorescence analysis of U-2 OS cells using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGelsolin Rabbit pAb
Catalog No.A16869
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments. Defects in this gene are a cause of familial amyloidosis Finnish type (FAF). Multiple transcript variants encoding several different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 282-438 of human Gelsolin (NP_000168.1).
SequenceAGTEDTAKEDAANRKLAKLYKVSNGAGTMSVSLVADENPFAQGALKSEDCFILDHGKDGKIFVWKGKQANTEERKAALKTASDFITKMDYPKQTQVSVLPEGGETPLFKQFFKNWRDPDQTDGLGLSYLSSHIANVERVPFDAATLHTSTAMAAQHG
Gene ID
Swiss Prot
SynonymsADF; AGEL; Gelsolin
Calculated MW86kDa
Observed MW86kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, Mouse lung, Rat lung
Cellular locationCytoplasm, Secreted, cytoskeleton.

Documents

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ABclonal:Western blot - Gelsolin Rabbit pAb (A16869)}

Western blot - Gelsolin Rabbit pAb (A16869)

Western blot analysis of various lysates using Gelsolin Rabbit pAb (A16869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - Gelsolin Rabbit pAb (A16869)}

Western blot - Gelsolin Rabbit pAb (A16869)

Western blot analysis of various lysates using Gelsolin Rabbit pAb (A16869) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)}

Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)}

Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)}

Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Gelsolin Rabbit pAb (A16869)}

Immunohistochemistry - Gelsolin Rabbit pAb (A16869)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - Gelsolin Rabbit pAb (A16869)}

Immunofluorescence - Gelsolin Rabbit pAb (A16869)

Immunofluorescence analysis of C6 cells using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Gelsolin Rabbit pAb (A16869)}

Immunofluorescence - Gelsolin Rabbit pAb (A16869)

Immunofluorescence analysis of NIH-3T3 cells using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Gelsolin Rabbit pAb (A16869)}

Immunofluorescence - Gelsolin Rabbit pAb (A16869)

Immunofluorescence analysis of U-2 OS cells using Gelsolin Rabbit pAb (A16869) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)