제품 > 항체 > 다중클론항체(pAb)

Ghd 7.1 Rabbit pAb (A20584)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Oryza sativa

ABclonal:Western blot - Ghd 7.1 Rabbit pAb (A20584)

Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Ghd 7.1 Rabbit pAb (A20584) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product nameGhd 7.1 Rabbit pAb
Catalog No.A20584
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Probable transcription factor involved in the regulation of flowering time under long day (LD) conditions. Functions as repressor of flowering. Controls flowering time by negatively regulating the expression of HD3A. Acts downstream of the phytochrome B to repress the expression of EHD1, an activator of the flowering promoter genes HD3A and RFT1. Controls photoperiodic flowering response. Seems to be one of the component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of PRR73, PRR37, PRR95, PRR59 and PPR1 result to circadian waves that may be at the basis of the endogenous circadian clock (By similarity).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of Oryza sativa Ghd 7.1 (Q0D3B6).
SequenceMMGTAHHNQTAGSALGVGVGDANDAVPGAGGGGYSDPDGGPISGVQRPPQVCWERFIQKKTIKVLLVDSDDSTRQVVSALLRHCMYEVIPAENGQQAWTY
Gene ID
Swiss Prot
SynonymsHD2; DTH7; PRR37; OsPRR37; Ghd 7.1
Calculated MW80kDa
Observed MW80kDa
ReactivityOryza sativa
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesspikes
Cellular locationnucleus
    ABclonal:Western blot - Ghd 7.1 Rabbit pAb (A20584)}

    Western blot - Ghd 7.1 Rabbit pAb (A20584)

    Western blot analysis of extracts of various tissues from the japonica rice (Oryza sativa L.) variety Zhonghua 11, using Ghd 7.1 Rabbit pAb (A20584) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)