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GluR2/GRIA2 Rabbit mAb (A11316)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:GluR2/GRIA2 Rabbit mAb
ABclonal:Western blot - GluR2/GRIA2 Rabbit mAb (A11316)

Western blot analysis of various lysates using GluR2/GRIA2 Rabbit mAb (A11316) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GluR2/GRIA2 Rabbit mAb (A11316)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using GluR2/GRIA2 Rabbit mAb (A11316) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - GluR2/GRIA2 Rabbit mAb (A11316)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using GluR2/GRIA2 Rabbit mAb (A11316) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - GluR2/GRIA2 Rabbit mAb (A11316)

Confocal imaging of paraffin-embedded Rat brain tissue using GluR2/GRIA2 Rabbit mAb (A11316, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - GluR2/GRIA2 Rabbit mAb (A11316)

Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2/GRIA2 Rabbit mAb (A11316, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product nameGluR2/GRIA2 Rabbit mAb
Catalog No.A11316
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC0572
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, GRIA1-4. The subunit encoded by this gene (GRIA2) is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to render the channel impermeable to Ca(2+). Human and animal studies suggest that pre-mRNA editing is essential for brain function, and defective GRIA2 RNA editing at the Q/R site may be relevant to amyotrophic lateral sclerosis (ALS) etiology. Alternative splicing, resulting in transcript variants encoding different isoforms, (including the flip and flop isoforms that vary in their signal transduction properties), has been noted for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 150-250 of human GluR2/GRIA2 (P42262).
SequenceYLYDSDRGLSTLQAVLDSAAEKKWQVTAINVGNINNDKKDEMYRSLFQDLELKKERRVILDCERDKVNDIVDQVITIGKHVKGYHYIIANLGFTDGDLLKI
Gene ID
Swiss Prot
SynonymsGLUR2; GLURB; GluA2; HBGR2; NEDLIB; gluR-2; gluR-B; GluR-K2; GluR2/GRIA2
Calculated MW99kDa
Observed MW99kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:100 - 1:800
  • IF/ICC 1:100 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Endoplasmic reticulum membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse.
Customer validation

WB(Rattus norvegicus, Mus musculus)

IF(Rattus norvegicus)

Documents

Certificate of Compliance

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ABclonal:Western blot - GluR2/GRIA2 Rabbit mAb (A11316)}

Western blot - GluR2/GRIA2 Rabbit mAb (A11316)

Western blot analysis of various lysates using GluR2/GRIA2 Rabbit mAb (A11316) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GluR2/GRIA2 Rabbit mAb (A11316)}

Immunohistochemistry - GluR2/GRIA2 Rabbit mAb (A11316)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using GluR2/GRIA2 Rabbit mAb (A11316) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - GluR2/GRIA2 Rabbit mAb (A11316)}

Immunohistochemistry - GluR2/GRIA2 Rabbit mAb (A11316)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using GluR2/GRIA2 Rabbit mAb (A11316) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - GluR2/GRIA2 Rabbit mAb (A11316)}

Immunofluorescence - GluR2/GRIA2 Rabbit mAb (A11316)

Confocal imaging of paraffin-embedded Rat brain tissue using GluR2/GRIA2 Rabbit mAb (A11316, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - GluR2/GRIA2 Rabbit mAb (A11316)}

Immunofluorescence - GluR2/GRIA2 Rabbit mAb (A11316)

Confocal imaging of paraffin-embedded Mouse brain tissue using GluR2/GRIA2 Rabbit mAb (A11316, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)