제품 > 항체 > 다중클론항체(pAb)

Glutaminase (GLS) Rabbit pAb (A3885)

Review (1)Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Glutaminase (GLS) Rabbit pAb (A3885)

Western blot analysis of extracts of various cell lines, using Glutaminase (Glutaminase (GLS)) antibody (A3885) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)

Immunofluorescence analysis of NIH/3T3 cells using Glutaminase (GLS) antibody (A3885) at dilution of 1:150. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)

Immunofluorescence analysis of PC-12 cells using Glutaminase (GLS) antibody (A3885) at dilution of 1:150. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)

Immunofluorescence analysis of SH-SY5Y cells using Glutaminase (GLS) antibody (A3885) at dilution of 1:150. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Glutaminase (GLS) Rabbit pAb(A3885)

Overview

Product nameGlutaminase (GLS) Rabbit pAb
Catalog No.A3885
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human Glutaminase (Glutaminase (GLS)) (NP_055720.3).
SequenceKVNPFPKDRWNNTPMDEALHFGHHDVFKILQEYQVQYTPQGDSDNGKENQTVHKNLDGLL
Gene ID
Swiss Prot
SynonymsGAC; GAM; KGA; GLS1; AAD20; DEE71; GDPAG; CASGID; EIEE71
Calculated MW73kDa
Observed MW65-73kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-251MG, NIH/3T3, Mouse brain, Rat kidney
Cellular locationCytoplasm, Mitochondrion, cytosol
Customer validation

(MCF-7)

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - Glutaminase (GLS) Rabbit pAb (A3885)}

Western blot - Glutaminase (GLS) Rabbit pAb (A3885)

Western blot analysis of extracts of various cell lines, using Glutaminase (Glutaminase (GLS)) antibody (A3885) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)}

Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)

Immunofluorescence analysis of NIH/3T3 cells using Glutaminase (GLS) antibody (A3885) at dilution of 1:150. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)}

Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)

Immunofluorescence analysis of PC-12 cells using Glutaminase (GLS) antibody (A3885) at dilution of 1:150. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)}

Immunofluorescence - Glutaminase (GLS) Rabbit pAb (A3885)

Immunofluorescence analysis of SH-SY5Y cells using Glutaminase (GLS) antibody (A3885) at dilution of 1:150. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A3885

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A3885? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)