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Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Western blot analysis of various lysates, using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunofluorescence analysis of HeLa cells using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameGlutamine Synthetase (GLUL) Rabbit pAb
Catalog No.A21822
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-373 of human Glutamine Synthetase (GLUL) (NP_002056.2).
SequenceMTTSASSHLNKGIKQVYMSLPQGEKVQAMYIWIDGTGEGLRCKTRTLDSEPKCVEELPEWNFDGSSTLQSEGSNSDMYLVPAAMFRDPFRKDPNKLVLCEVFKYNRRPAETNLRHTCKRIMDMVSNQHPWFGMEQEYTLMGTDGHPFGWPSNGFPGPQGPYYCGVGADRAYGRDIVEAHYRACLYAGVKIAGTNAEVMPAQWEFQIGPCEGISMGDHLWVARFILHRVCEDFGVIATFDPKPIPGNWNGAGCHTNFSTKAMREENGLKYIEEAIEKLSKRHQYHIRAYDPKGGLDNARRLTGFHETSNINDFSAGVANRSASIRIPRTVGQEKKGYFEDRRPSANCDPFSVTEALIRTCLLNETGDEPFQYKN
Gene ID
Swiss Prot
SynonymsGS; GLNS; PIG43; PIG59; Glutamine Synthetase (GLUL)
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat liver, Rat brain
Cellular locationCytoplasm, Mitochondrion.

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ABclonal:Western blot - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Western blot - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Western blot analysis of various lysates, using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunohistochemistry - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at a dilution of  1:1000 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunofluorescence analysis of HeLa cells using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)}

Immunofluorescence - Glutamine Synthetase (GLUL) Rabbit pAb (A21822)

Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase (GLUL) Rabbit pAb (A21822) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (2)

Secondary Antibodies (26)