Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Gm13125 Rabbit pAb (A12619) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Gm13125 Rabbit pAb (A12619) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human stomach using Gm13125 Rabbit pAb (A12619) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
| Product name | Gm13125 Rabbit pAb |
|---|---|
| Catalog No. | A12619 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-477 of mouse Gm13125 (NP_001108549.1). |
|---|---|
| Sequence | MSSKPLLTLQELAAQNILRNEALAISALQYLPELFLPQLLKQAYDGNHLKVLRAIVSSWPFPRLPLGALKKRTPYLETQLQVVLEEVDKLLIQEVHPREYKLEVLDLRSVGQNYLNVWPGAMDDWLPKTTQTDPCCSKTVMTQPLKVLVDLQLNNRGQSRLFSYLVQWSDRRKGLLQLYCNKLQIWSPSQQNYRKLSQKVNLDYVETLGLHSSCSPSFLLNFAPYLRQMRNLRKLALSNIREEHIISPEKRRCIITRFTLQILKLESLQKLHLDAVCFLEGHLDQLLGSVKTSLDELAVTHCNLSVSEWSHFSEFPCVSQLQQLNLENVRLTGLSPEPLQVLLVKAGPTLVALDLEDCHLEDGQLYAILPALSKCFKLTKFSFYGNQISMLAMKDLLHHTASLIHLRLELYPVPQDSYDYHGTPLMQRMQQHCDELMNLLKTFRNPGRVFFGTDRCDQCCNRYIYNKTIMCNCRRSY |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Gm13125; EG627009; Pramef20 |
| Calculated MW | 55kDa |
| Observed MW | 55kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry |
| Positive samples | Mouse heart, Rat heart |
| Cellular location | cytoplasm |
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