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GnRH1 Rabbit pAb (A25429)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Western blot - GnRH1 Rabbit pAb (A25429)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GnRH1 using GnRH1 Rabbit pAb(A25429) at 1:1000 dilution. 
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameGnRH1 Rabbit pAb
Catalog No.A25429
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a preproprotein that is proteolytically processed to generate a peptide that is a member of the gonadotropin-releasing hormone (GnRH) family of peptides. Alternative splicing results in multiple transcript variants, at least one of which is secreted and then cleaved to generate gonadoliberin-1 and GnRH-associated peptide 1. Gonadoliberin-1 stimulates the release of luteinizing and follicle stimulating hormones, which are important for reproduction. Mutations in this gene are associated with hypogonadotropic hypogonadism.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-92 of human GNRH1(NP_001076580.1).
SequenceMKPIQKLLAGLILLTWCVEGCSSQHWSYGLRPGGKRDAENLIDSFQEIVKEVGQLAETQRFECTTHQPRSPLRDLKGALESLIEEETGQKKI
Gene ID
Swiss Prot
SynonymsGRH; GNRH; LHRH; LNRH
Calculated MW10kDa
Observed MW12kDa
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T transfected with GnRH1
Cellular locationSecreted

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    ABclonal:Western blot - GnRH1 Rabbit pAb (A25429)}

    Western blot - GnRH1 Rabbit pAb (A25429)

    Western blot analysis of lysates from wild type (WT) and 293T cells transfected with GnRH1 using GnRH1 Rabbit pAb(A25429) at 1:1000 dilution. 
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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