제품 > 항체 > 다중클론항체(pAb)

Gsdmc3 Rabbit pAb (A16741)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates using Gsdmc3 Rabbit pAb (A16741) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Overview

Product name	Gsdmc3 Rabbit pAb
Catalog No.	A16741
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Predicted to enable phosphatidylinositol-4, 5-bisphosphate binding activity; phosphatidylinositol-4-phosphate binding activity; and phosphatidylserine binding activity. Predicted to be involved in defense response to bacterium and pyroptosis. Predicted to act upstream of or within programmed cell death. Predicted to be located in cytoplasm. Is expressed in gut and metanephros. Orthologous to human GSDMC (gasdermin C).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 2-80 of mouse Gsdmc3 (NP_899017.2).
Sequence	GYSFDRASKDVVKKLQGRDLRPVECLSDATKFRLFHILQETPRSGWETEDIPVGFTLLDLLEPNFPVPEPEVSAPKPFI
Gene ID
Swiss Prot
Synonyms	9930109F21Rik; Gsdmc3
Calculated MW	54kDa
Observed MW	54kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	Mouse spleen, Mouse brain
Cellular location	Cytoplasm
Customer validation	WB(Mus musculus) ELISA(Mus musculus)